All potential runners in the mass participation event will have three gender options when the ballot opens on October 1st - male, female and non-binary.
The elite races, which all operate under World Athletics rules, will not offer a non-binary gender option.
"This is a significant step forward for the TCS London Marathon as we continue our journey to make our event truly inclusive," Event Director Hugh Brasher said.
"We know there is still much more to be done, but changes such as this demonstrate our commitment to making the TCS London Marathon an event that is for everyone."
The change has been implemented following an extensive review and consultation period by London Marathon Events (LME) aimed at making the event the most "diverse, equitable and inclusive" marathon in the world, the statement added.
The 2023 London Marathon will take place on April 23.
The three gender options (male, female and non-binary) will be offered to all participants registering for the mass participation element of the TCS London Marathon via other entry routes, including charity entries.
This change to the entry system will also be implemented across the mass participation element of all events organised by LME.
