About 70 Spanish supporters were attacked by about 80 Lazio supporters in Via Leonina, near the Colosseum, at about 23:00 CET on Wednesday, a Rome police spokesperson said.
They used blunt objects and knives, police said, releasing a picture of weapons presumed to have been used in the attack, including a hammer, sticks and batons, wrenches and a chain.
The attackers fled as the police arrived, the spokesperson said.