Francesco Acerbi, Alessandro Bastoni, Nicolo Barella, Matteo Darmian and Federico Dimarco, who were not in last week's preliminary list, will join the Italy training camp in Florence on Sunday, a day after Inter face Manchester City for the European Cup.
Mancini dropped Federico Baschirotto, Alessandro Buongiorno, Alessandro Florenzi, Federico Gatti, Manuel Locatelli and Mattia Zaccagni, who will leave the squad on Friday.
Monza's Matteo Pessina and Sassuolo's Domenico Berardi left the training camp with injuries.
Italy travel to the Netherlands on June 14th to face Spain the next day and will next play for either third place or the Nations League title four days later.
Squad:
Goalkeepers: Gianluigi Donnarumma (Paris St Germain), Alex Meret (Napoli), Guglielmo Vicario (Empoli)
Defenders: Francesco Acerbi, Alessandro Bastoni, Matteo Darmian, Federico Dimarco (all Inter Milan), Giovanni Di Lorenzo (Napoli), Leonardo Spinazzola (AS Roma), Rafael Toloi (Atalanta), Leonardo Bonucci (Juventus)
Midfielders: Nicolo Barella (Inter Milan), Bryan Cristante (AS Roma), Lorenzo Pellegrini (AS Roma), Davide Frattesi (Sassuolo), Jorginho (Arsenal), Marco Verratti (Paris St Germain), Nicolo Zaniolo (Galatasaray)
Forwards: Federico Chiesa (Juventus), Wilfried Gnonto (Leeds United), Ciro Immobile (Lazio), Giacomo Raspadori (Napoli), Mateo Retegui (Tigre).
