Arsenal's Timber will not travel with the Dutch to Valencia for Sunday’s second leg.
They will also have to do without 19-year-old defender Jorrel Hato, suspended after being red-carded late in Thursday’s game. Netherlands coach Ronald Koeman has added Aston Villa defender Ian Maatsen to his squad as cover.
Maatsen received a similar emergency call-up during last year’s European Championship but is still to win a first cap.